Examples of PBS in a sentence
The culture medium was removed and the xxxxx were washed three times with PBS at 37ºC to eliminate unattached cells.
Cells were washed in PBS between each incubation step before flow cytometric analysis of the cells.
Cells were washed in PBS between incubation steps and propidium iodide added before flow cytometric analysis of the cells.
The culture medium was removed, the xxxxx were washed three times with PBS at 37ºC and were filled with 2 ml of 0.1% sodium lauryl sulfate (Sigma).
Analysis of VLR-C8 idiotope specificity for BCL1 BCR For flow cytometric analysis, the scFv transfected 293T were sequentially incubated with 0.04 mg/ml purified VLR protein, followed by Alexa Fluor 647- conjugated 8A5 anti-VLRB mAb and Anti-Myc Tag, clone 4A6, Alexa Fluor 488 conjugate (Millipore) and 7-AAD to verify cell surface expression of transfected scFv; cells were washed in PBS between each incubation step.